RNAfold 
 
Class 
G::Seq::FreeEnergy; 
 
 
Function 
executes RNA fold 
 
Description 
Using RNAfold, this method calculates the nucleotide's free energy, and 
estimates the secondary structure.   
The calculation is executed for a specified region. 
 
Requirements 
modules   
SubOpt.pm 
 
applications 
none 
 
data 
none 
 
Parameters 
essential 
 
first     pointer   Genome            G's instance 
second    int       start_position    The starting position 
third     int       end_position      The ending position 
 
option 
output    string  [stdout or f]    default=stdout 
filename  string  file_name        default=FreeEnergy.csv 
 
Returns 
none 
 
 
Usage 
&RNAfold(pointer Genome,  int start_position,  int end_position,  
 
-output=>'stdout', -filename=>'FreeEnergy.csv'); 
 
Input file  
none 
 
Output file  
csv file in text format 
graph in ps format  
 
References 
none  
 
Comments 
none 
 
 
Authors 
This method was written by Naoki Okubo(@g-language.org) at Institute for  
 
Advanced Biosciences Keio Univ.  
 
 
History